Reprolife Co., Ltd.


In slow freezing, which was the main technique for cell freezing in the past, cells immersed into cryoprotectants are slowly cooled to a specific temperature to dehydrate and freeze cells by a difference in osmotic pressure due to ice crystals that occurred extracellularly. This procedure requires a dedicated device called a “program freezer” and a prolonged time of working. Compared to the slow freezing, vitrification requires no specific devices and much shorter time for the procedure, resulting in widespread use of this technique.