Achieve Excellent Survival Rates with the Cryotec Method

The Cryotec Method enables the safe freezing and thawing of oocytes and embryos at all stages and promises exceptional survival rates post-thawing. Reprolife began supplying its products internationally in 2012. At Reprolife, we believe that safely storing oocytes and embryos and protecting them from damage until they are needed is the aim of the freezing and thawing processes. The Cryotec Method consistently achieves this goal.

Cryotec Products

Cryotec

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Cryopreservation solutions

Vitrification Solutions

One of the major causes of damage to oocytes and embryos during vitrification is moving on to the next step before reaching equilibrium. In the past, embryologists relied on their experience to determine whether equilibrium had been reached, however, a specific gravity difference in Reprolife’s vitrification solution means that anyone can easily accurately determine whether equilibrium has been attained. At the same time, toxicity is also reduced. Reprolife strives to make this procedure, which must be conducted under a time limit, as simple and stress-free as possible.
Thawing solution

Warming Solutions

A major issue when thawing has been the effect that sudden changes in osmotic pressure can have on oocytes and embryos. The over-expansion (hyper-hydration) that can occur as a result of these changes can cause damage to cells and cytoskeletons. Cryotec Preservation minimizes the difference in osmotic pressure of the various solutions while adding hydroxypropyl cellulose (HPC). This increases viscosity and achieves a gradual and safe dilution.

Plates

Reprolife has developed the world’s first specialized freezing and thawing plate. This innovation simplifies manipulation while also making it possible to check oocytes and embryos without looking away from the microscope. The result is a more secure operating procedure.

  • Vitri PlateVitri Plate
  • Warm PlateWarm Plate
  • Vitri Plate
  • Warm Plate
  • We have developed the world's first specialized vitrification plate. Designed to enable safer oocytes and embryos manipulation.
  • By placing the straw (Cryotec) onto the grooves built into the plate, we have made the loading operation extremely simple.
  • We adjusted the height and size to make it easier to conduct the manipulation with minimal microscope operation.
  • Using a round-bottom well eliminates shadows caused by light refraction, making it easy to follow the oocytes and embryos.
  • Placing the waste fluid drains on the side of the well simplifies operability.
  • When bringing the previous solution to the bottom of the well, the round-bottom shape helps prevent the raveling of liquid build-up, enabling extremely slow dilution which is gentle on the oocytes and embryos.

Straw [Cryotec]

The handles of Reprolife’s straws are thicker, improving operability and also making it easier to record patient information. Reprolife has also developed a thin sheet with an optimal width which reduces the frequent occurrence of bubbles during the thawing process. The Reprolife straw boasts exceptional ease of use. Straw [Cryotec]

Advantages of Cryotec

    • The thin sheet (0.1mm) with an optimal width (1.4mm) reduces the occurrence of bubbles, maximizing freezing and thawing speeds.
    • The right-angled triangle on the edge of each straw eliminates misplacement during operation.
    • The square shape of the main unit stabilizes operation, making it easier to record patient information.

Protocol

Vitrification

One of the major causes of damage to oocytes and embryos during vitrification is moving on to the next step before reaching equilibrium. In the past, embryologists relied on their experience to determine whether equilibrium had been reached, however, a specific gravity difference in Reprolife’s vitrification solution means that anyone can accurately determine whether equilibrium has been attained. At the same time, toxicity is reduced. The result is a reduction in the stress of operating under a time limit.

Cryopreservation solutions YouTube

Warming

A major issue when thawing has been the effect that sudden changes in osmotic pressure can have on oocytes and embryos. The over-expansion (hyper-hydration) that can occur as a result of these changes can cause damage to cells and cytoskeletons. Cryotec Preservation minimizes the difference in osmotic pressure of the various solutions while adding hydroxypropyl cellulose (HPC). This increases viscosity and achieves a gradual and safe dilution.

Thawing solution YouTube

Certificate of Analysis

Our corporate philosophy inspires us to develop and manufacture the safest and most effective medical products and to provide them at fair prices in order to help patients around the world struggling with fertility issues. In order to achieve this, we ship only products that have undergone thorough quality control, and which have passed multiple rigorous tests. You can always use Reprolife products with complete peace of mind.

Science Hotline

We are always happy to answer questions related to the Reprolife range of products, the Cryotec Method, and freezing and thawing. We also welcome scientific inquiries. Please feel free to contact us about even the most minor of matters. Please contact us by filling out the inquiry form. The relevant representative will be in touch with you shortly.

Achieve Excellent Survival Rates with the Cryotec Method

To attain such exceptional results, in addition to ordinary tests, Reprolife conducted survivability tests on 30 porcine oocytes (oocytes from pigs), considered to be the most difficult mammalian oocytes to successfully vitrify. Reprolife offers only products that achieved significant survival rates in these tests.

Advantages of the Cryotec Method

Quality Control

 

Vitrification solutions

Cryopreservation solutions

Advantages of Cryotec Vitrification solutions

Warming solutions

Thawing solution

Advantages of Cryotec Warming Solutions

Plates

  • Vitri PlateVitri Plate
  • Warm PlateWarm Plate
  • Vitri Plate
  • Warm Plate

Straw [Cryotec]

Straw [Cryotec]
Vitrification Kit 101(for 3 patients/up to 3 oocytes/embryos per application)
Vitrification Kit 101(for 3 patients/up to 3 oocytes/embryos per application)

・1.0ml Equilibration Solution(ES)x1
・1.0ml Vitrification Solution(VS )x2
・4 Cryotecs
・3 Vitri Plates
Shelf-life:1 year at 2°C~8°C

Warming Kit 102(for 1 patient/up to 3 oocytes/embryos)
Warming Kit 102(for 1 patient/up to 3 oocytes/embryos)

・1.8ml Thawing Solution(TS)x1
・0.5ml Diluent Solution(DS)x1
・0.5ml Washing Solution(WS)x2
・1 Warm Plate
Shelf-life:1 year at 2°C~8°C

Vitrification Solution 110(for 10 applications)
Vitrification Solution 110(for 10 applications)

・1.8ml Equilibration Solution(ES)x2
・1.8ml Vitrification Solution(VS)x4
Shelf-life:1 year at 2°C~8°C

Warming Solution 205(for 5 applications)
Warming Solution 205(for 5 applications)

・1.8ml Thawing Solution(TS)x5
・1.8ml Diluent Solution(DS)x1
・1.8ml Washing Solution(WS)x2
Shelf-life:1 year at 2°C~8°C

Cryotec(10 straws)
Cryotec(10 straws)

・10 Cryotecs in uniform color.
*Choose from red, white, yellow, blue, and green.
Shelf-life:2 years

Vitri Plate(Set of 10)
Vitri Plate(Set of 10)

・10 plates
Shelf-life:2 years

Warm Plate(Set of 10)
Warm Plate(Set of 10)

・10 plates
Shelf-life:2 years

Cooling Rack(Compact liquid nitrogen container)
Cooling Rack(Compact liquid nitrogen container)

・1pc(W20cmxD11cmxH11cm)

Products