Reprolife Co., Ltd.

Products

Cryotec method, achieving excellent survival rate

Cryotec method enables safe freezing and thawing of oocytes and embryos at any stage and promises an excellent survival rate after thawing.
We have started supplying globally in 2012.
We believe that the goal of freezing and thawing is to "safely store precious oocytes and embryos until they are needed without damaging or endangering them," and Cryotec method achieves this.

Cryotec Products

Cryotec

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Cryopreservation solutions

Vitrification solutions

One of the major causes of damage to oocytes and embryos in vitrification is moving on to the next step before completing equilibrium.
While in the past determining equilibrium completion was based on the embryologist's experience, we have added a specific gravity difference to the solution so that anyone can accurately determine equilibrium completion, while also reducing toxicity among other things to relieve the pressure when operating under a time limit.

Thawing solution

Warming solutions

A major issue when thawing has been the fact that sudden changes in osmotic shock lead to over-expansion (hyper-hydration) in the oocytes and embryos, causing damage to the cells and cytoskeletons.
Cryotec preservation is a method which minimizes osmotic shock differences in the various solutions while adding HPC, thereby increasing viscosity and achieving extremely slow and safe dilution.

Plate

We have developed the world's first specialized freezing and thawing plate.
This simplifies the enforcement of manipulation while also making it possible to check the oocyte without looking away from the microscope, enabling a more secure operation.

  • We have developed the world's first specialized vitrification plate. Designed to enable safer oocytes and embryos manipulation.
  • By placing the straw (Cryotec) onto the grooves built into the plate, we have made the loading operation extremely simple.
  • We adjusted the height and size to make it easier to conduct the manipulation with minimal microscope operation.
  • Using a round-bottom well eliminates shadows caused by light refraction, making it easy to follow the oocytes and embryos.
  • Placing the waste fluid drains on the side of the well simplifies operability.
  • When bringing the previous solution to the bottom of the well, the round-bottom shape helps prevent the raveling of liquid build-up, enabling extremely slow dilution which is gentle on the oocytes and embryos.

Straw [Cryotec]

By making the handles thicker, we have not only improved operability but also made it easier to write down patient information.
We also developed the thin sheet with the optimal width to reduce the frequent occurrence of bubbles when thawing. It is the most "easy-to-operate" straw.

Straw [Cryotec]

Advantages of Cryotec

  • Using the thin sheet (0.1mm) with an optimal width (1.4mm) reduces the occurrence of bubbles and maximizing the freezing and thawing speeds.
  • Developing the edges of the sheet right triangles eliminates misplacement when operation.
  • By making the main unit square-shaped, we stabilized operation and made it easier to write down patient information.

Vitrification

One of the major causes of damage to oocytes and embryos in vitrification is moving on to the next step before completing equilibrium. While in the past determining equilibrium completion was based on the embryologist's experience, we have added a specific gravity difference to the solution so that anyone can accurately determine equilibrium completion, while also reducing toxicity among other things to relieve the pressure when operating under a time limit.

Protocol

Vitrification

Cryopreservation solutions YouTube

Warming

Thawing solution YouTube

Warming

A major issue when thawing has been the fact that sudden changes in osmotic shock lead to over-expansion (hyper-hydration) in the oocytes and embryos, causing damage to the cells and cytoskeletons. Cryotec preservation is a method which minimizes osmotic shock in the various solutions while adding HPC, thereby increasing viscosity and achieving extremely slow and safe dilution.

Cryotec Method Protocol

Certificate of Analysis

To achieve our corporate philosophy of
"developing and manufacturing the most effective and safest medical products and providing them at the lowest prices to help patients around the world with fertility issues,"
we only ship products which have undergone thorough quality control and passed multiple rigorous tests. Please use them with peace of mind.   

Science hotline

We, Reprolife always accept questions relating to Cryotec method, freezing and thawing as well as scientific inquiries.
Please feel free to inquire with us even about the most minor matters.

Please send your inquiry by filling out the inquiry form.
Our sales representative will get back to you.