Achieve Excellent Survival Rates with the Cryotec Method
The Cryotec Method enables the safe freezing and thawing of oocytes and embryos at all stages and promises exceptional survival rates post-thawing.
REPROLIFE began supplying its products internationally in 2012.
At REPROLIFE, we believe that safely storing oocytes and embryos and protecting them from damage until they are needed is the aim of the freezing and thawing processes. The Cryotec Method consistently achieves this goal.
Cryotec Products
Vitrification Solutions
One of the major causes of damage to oocytes and embryos during vitrification is moving on to the next step before reaching equilibrium.
In the past, embryologists relied on their experience to determine whether equilibrium had been reached, however, a specific gravity difference in REPROLIFE’s vitrification solution means that anyone can easily accurately determine whether equilibrium has been attained. At the same time, toxicity is also reduced.
REPROLIFE strives to make this procedure, which must be conducted under a time limit, as simple and stress-free as possible.
Warming Solutions
A major issue when thawing has been the effect that sudden changes in osmotic pressure can have on oocytes and embryos. The over-expansion (hyper-hydration) that can occur as a result of these changes can cause damage to cells and cytoskeletons.
Cryotec Preservation minimizes the difference in osmotic pressure of the various solutions while adding hydroxypropyl cellulose (HPC). This increases viscosity and achieves a gradual and safe dilution.
Plates
REPROLIFE has developed our specialized freezing and thawing plate in 2012. This innovation simplifies manipulation while also making it possible to check oocytes and embryos without turning away from the microscope. The result is a more secure operating procedure.
Vitri Plate
Warm Plate
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REPROLIFE also developed our specialized vitrification plate in 2012 specially designed to enable safer oocyte and embryo manipulation.
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Loading is made extremely simple owing to built-in grooves in which to place the straw (Cryotec).
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Height and size adjustments improve ease of manipulation with minimal microscope operation.
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Round-bottom wells eliminate shadows caused by light refraction, making it harder to lose sight of oocytes and embryos.
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Operability is simplified due to waste-fluid drains on the side of wells.
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The round-bottomed shape of wells helps prevent the raveling of liquid build-up when bringing the previous solution to the bottom of the well, enabling exceptionally slow dilution, which is gentle on oocytes and embryos.
Straw [Cryotec]
The thick handle does not only improve operability, but also makes it easier to fill in patient information within the straws. In addition, REEPROLIFE also developed the thin sheet with optimal width to suppress the generation of air bubbles that occur frequently during thawing. The freezing container is also very easy to operate.
![Straw [Cryotec]](https://global.reprolife.jp/wp-content/uploads/2021/06/cryotec_touketu.png)
Advantages of Cryotec
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- The thin sheet (0.1mm) with an optimal width (1.4mm) reduces the occurrence of bubbles, maximizing freezing and thawing speeds.
- The right-angled triangle on the edge of each straw eliminates misplacement during operation.
- The square shape of the main unit stabilizes operation, making it easier to record patient information.
Protocol
Vitrification
One of the major causes of damage to oocytes and embryos during vitrification is moving on to the next step before reaching equilibrium.
In the past, embryologists relied on their experience to determine whether equilibrium had been reached, however, a specific gravity difference in REPROLIFE’s vitrification solution means that anyone can accurately determine whether equilibrium has been attained. At the same time, toxicity is reduced. The result is a reduction in the stress of operating under a time limit.
Warming
A major issue when thawing has been the effect that sudden changes in osmotic pressure can have on oocytes and embryos. The over-expansion (hyper-hydration) that can occur as a result of these changes can cause damage to cells and cytoskeletons.
Cryotec Preservation minimizes the difference in osmotic pressure of the various solutions while adding hydroxypropyl cellulose (HPC). This increases viscosity and achieves a gradual and safe dilution.
Certificate of Analysis
Our corporate philosophy inspires us to develop and manufacture the safest and most effective medical products and to provide them at fair prices in order to help patients around the world struggling with fertility issues.
In order to achieve this, we ship only products that have undergone thorough quality control, and which have passed multiple rigorous tests.
You can always use REPROLIFE products with complete peace of mind.
Science Hotline
We are always happy to answer questions related to the REPROLIFE range of products, the Cryotec Method, and freezing and thawing. We also welcome scientific inquiries. Please feel free to contact us about even the most minor of matters.
Please contact us by filling out the inquiry form. The relevant representative will be in touch with you shortly.
Achieve Excellent Survival Rates with the Cryotec Method
To attain such exceptional results, in addition to ordinary tests, REPROLIFE conducted survivability tests on 30 porcine oocytes (oocytes from pigs), considered to be the most difficult mammalian oocytes to successfully vitrify. REPROLIFE offers only products that achieved significant survival rates in these tests.
Advantages of the Cryotec Method
- The Cryotec Method’s thawing system can also be used on oocytes and embryos frozen using other vitrification methods, enabling an even higher survival rate.
- The Cryotec Method is comparatively simpler, enabling safe and easy freezing, and, as a result, better outcomes.
- The Cryotec Method’s solutions are very mild, so they can be used with lower-grade oocytes and embryos that were difficult to freeze using traditional methods.
- The Cryotec Method allows embryos and oocytes to be frozen using the same protocol, providing peace of mind.
Quality Control
- pH test
- Osmometry
- Postovulatory aging (POA) testing
Our products have a proven 100-percent survivability rate using porcine oocytes.
- Mouse embryo assay
- Endotoxin test
Vitrification solutions
Advantages of Cryotec Vitrification solutions
- Solutions are safe and long-lasting because all media are free of blood serum and protein. (12-month shelf life)
- REPROLIFE uses very low-endotoxin trehalose solution to increase safety and achieve a high degree of vitrification. Because of this consideration, our product reduces the impact on the cells.
- Safety is further increased by decreasing the density and chemical toxicity of the cryoprotectant.
- A unified protocol for oocytes in all stages is made possible by slowing osmotic changes as much as possible in order to reduce the burden on oocytes and embryos.
Warming solutions
Advantages of Cryotec Warming Solutions
- By adding the optimal amount of HPC, the viscosity of the solution is increased, and osmotic shock is reduced.
- By adjusting the speed of hydration and dehydration, damage caused by hyper hydration is prevented, enabling a safe recovery.
Plates
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- REPROLIFE also developed our specialized vitrification plate in 2012 specially designed to enable safer oocyte and embryo manipulation.
- Loading is made extremely simple owing to built-in grooves in which to place the straw (Cryotec).
- Height and size adjustments improve ease of manipulation with minimal microscope operation.
- Round-bottom wells eliminate shadows caused by light refraction, making it harder to lose sight of oocytes and embryos.
- Operability is simplified due to waste-fluid drains on the side of wells.
- The round-bottomed shape of wells helps prevent the raveling of liquid build-up when bringing the previous solution to the bottom of the well, enabling exceptionally slow dilution, which is gentle on oocytes and embryos.
- Vitri Plate
- Warm Plate
Straw [Cryotec]
- Using the thin sheet (0.1mm) with an optimal width (1.4mm) reduces the occurrence of bubbles and maximizing the freezing and thawing speeds.
- Developing the edges of the sheet right triangles eliminates misplacement when operation.
- By making the main unit square-shaped, we stabilized operation and made it easier to write down patient information.
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Vitrification Kit 101(for 3 patients/up to 3 oocytes/embryos per application)
・1.0ml Equilibration Solution(ES)x1
・1.0ml Vitrification Solution(VS )x2
・4 Cryotecs
・3 Vitri Plates
Shelf-life：1 year at 2°C～8°C
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Warming Kit 102(for 1 patient/up to 3 oocytes/embryos)
・1.8ml Thawing Solution(TS)x1
・0.5ml Diluent Solution(DS)x1
・0.5ml Washing Solution(WS)x2
・1 Warm Plate
Shelf-life：1 year at 2°C～8°C
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Vitrification Solution 110(for 10 applications)
・1.8ml Equilibration Solution(ES)x2
・1.8ml Vitrification Solution(VS)x4
Shelf-life：1 year at 2°C～8°C
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Warming Solution 205(for 5 applications)
・1.8ml Thawing Solution(TS)x5
・1.8ml Diluent Solution(DS)x1
・1.8ml Washing Solution(WS)x2
Shelf-life：1 year at 2°C～8°C
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Cryotec(10 straws)
・10 Cryotecs in uniform color.
*Choose from red, white, yellow, blue, and green.
Shelf-life：2 years
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Vitri Plate(Set of 10)
・10 plates
Shelf-life：2 years
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Warm Plate(Set of 10)
・10 plates
Shelf-life：2 years
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Cooling Rack(Compact liquid nitrogen container)
・1pc(W20cmxD11cmxH11cm)
