Reprolife Co., Ltd.

What is vitrification?

The vitrification method, which is one of many cell freezing methods, has currently spread all over the world as the easiest-to-use and safest technique. It can cryopreserve oocytes and embryos for many years safely without significant deterioration theoretically. It has played an important role to “stop the time of oocytes and embryos until the precious day that would eventually come,” as a means of fertility preservation and treatment for sterility.

History of vitrification

1949
Polge
Successful cryopreservation for a chicken’s spermatozoa with glycerol--Discovery of cryoprotectant
1972
Whittingham
First successful cryopreservation of a mouse embryo by slow freezing
1977
Willadsen
Establishment of a simplified slow freezing method
1983
Trounson
First reported case of human pregnancy/delivery with a cryopreserved embryo
1985
Rall,Fahy
First successful embryo freezing by a vitrification method
1991
Kuwayama
Successful vitrification of a bovine embryo by the 16-step method
1999
Kuwayama
First successful vitrification of a human oocyte by the minimum volume cooling (MVC) technique
2000
Kuwayama
Establishment of Cryotop Method
2003, 2004
Kuwayama
First successful pregnancy/delivery with cryopreserved oocyte in the United States, South America, and Europe
2012
Kuwayama
Establishment of Cryotec Method

Mechanism of vitrification

As it is often said that approximately 60 % of the human body is made from water, cells contain plenty of water. Water is the only substance in nature for which the volume increases when it is solidified.